3D models of reconstructed skin
TISSUE ENGINEERING AS A TOOL FOR EFFICACY
SILAB has patented SILABSKIN®, a brand revealing the company’s expertise in the technology of 3D models of reconstructed epidermis (SILABSKIN® RE) or reconstructed skin (SILABSKIN® FT).
These in vitro models were developed using primary cells obtained from skin biopsies, for which a great deal of information has been collected and entered is included in an in-house database (age, sex, phototype, stage of pre- or post-menopause, etc.).
The methodologies implemented are robust and SILAB is committed to three levels of requirements: traceability of the biological material, in-house control of skills and methods, as well as the quality and reproducibility of the models.
These multilayer models are the relevant base for demonstrating the mechanism of action of active ingredients at several levels prior to tests of clinical efficacy.
INNOVATE TO OBTAIN SKIN MODELS AS CLOSE AS POSSIBLE TO REALITY
Consistent with its strategy of innovation, SILAB has established partnerships with international universities. The results of this basic research have contributed to the most recent scientific progress and have further increased knowledge by identifying the molecular, cellular and tissue components involved in skin disorders.
These scientific discoveries have enabled SILAB to perfect its 3D models in order to mimic skin as close as possible to reality by proposing vascularized reconstructed skin or pigmented reconstructed epidermis.
Research conducted jointly with the University of Laval Quebec has recently led to the development of a cell extraction technique to isolate resident skin cells from the same human donor that also preserves immune and endothelial cells. This approach has resulted in the reconstruction of an autologous, vascularized and immunocompetent model of skin.
“HEALTHY OR COMPROMISED” SKIN MODELS
The experts of SILAB’s in vitro platform have developed 3D models to determine the biological consequences of different types of stress to which the skin may be exposed (aging, photoaging, altered barrier function, polluted environment, nutritional or physiological deficiency, etc.).
Attention paid to fragile skin, such as that with atopic dermatitis, an inflammatory cutaneous pathology, has led to the development of a 3D model mimicking the principal features of this skin pathology.
More recently, SILAB has devoted work to acneic skin and has developed a 3D model combining the two principal factors responsible for this pathology, modification of the composition of sebum and invasion by Cutibacterium acnes.
 Attiogbe et al., An in vitro autologous, vascularized, and immunocompetent Tissue Engineered Skin model obtained by the self-assembled approach, Acta Biomater., 2023.
 Laclaverie et al., Highlighting of two distinct cellular behaviors in skin from aged healthy volunteers, IFSCC Magazine, 2017
 Rouaud-Tinguely et al., From the morphologic to the transcriptomic characterization of a compromised 3D in vitro model mimicking atopic dermatitis, British Journal of Dermatology, 2015.
 Laclaverie et al., Development and characterization of a 3D in vitro model mimicking acneic skin, Experimental dermatology, 2020.
Laclaverie et al., Highlighting of two distinct cellular behaviors in skin from aged healthy volunteers, IFSCC Magazine, 2017
SELECTION OF ACTIVE INGREDIENTS
This expertise is at the origin of the development of innovative natural active ingredients that provide the skin with multiple cosmetic benefits.